Journal: Antioxidants

Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology

doi: 10.3390/antiox12010181

Figure Lengend Snippet: Antioxidant alters expression of p−STAT4 and p−mTOR from SLE CD8 + T cells. CD8 + T cells were isolated from the PBMCs of SLE patients and treated with 2.5 μM menadione with or without NAC for 4 h and processed according to the protocol mentioned in the method section. Representative figure showing no modulation in p−STAT3 expression when treated with PMA/Ion, menadione (2.5 μM) or NAC (5 Mm) ( A ). Representative figures showing a reduction in p−STAT4 and p−mTOR following NAC (5 mM) treatment compared to their activation by PMA/Ion or treatment with menadione (2.5 μM) ( B , C ). Cumulative graphical representation showing changes in p−STAT3, p−STAT4 and p−mTOR after menadione and NAC treatment ( D – F ). Error bar indicates SEM. The experiments were done from 15 SLE patients ( n = 10) for p-STAT assay and ( n = 5) for p−mTOR assay, and all samples were tested for significance by using one-way ANOVA, followed by Tukey’s multiple comparison. p < 0.05 was considered statistically significant (*); p < 0.0001 was considered extremely significant (****), and ns means non-significant.

Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ].

Techniques: Expressing, Isolation, Activation Assay